Optical Imaging Center

The imaging center of IBMS provides anexpertise in light, fluorescent and laser-scanning microscopies. This technologic platform develops and gives access to high-tech imaging technologies. It enables researchers the possibility of an integrated analysis of their models through different resolutions, from fine cellular structures toin vivo complex function of organs.

Conventional microscopes

NICON90I Upright fluorescence microscope

Samples: in-situs and histological sections, livemicro-organisms

Filters: light, dapi, fitc and tritc.

Objectives: 10X, 20X, 40X and 100X.

NICONTi Inverted fluorescence microscope

Samples: in-situs / histological sections, live micro-organisms, culturedcells.

Filters: DIC, dapi, fitc and tritc.

Objectives: 10X, 20X and 40X

OlympusSZX16 Zoom stereo microscope

Samples: live micro-organisms organs and small animals, cultured cells.

Filters: light, fitc and tritc.

Zoomratio: 16.4:1

Resolution: >900 l/mm

Laser-scanningmicroscopes

NICONA1 confocal laser-scanning microscope

The A1 is capable of capturing high-quality confocal images of cells, molecular events at high speed and enhanced sensitivity, 2D analysis and 3D volume rendering. The wavelengths of the lasers are 405 nm, 457nm, 477nm, 488nm, 514nm, 543nm and 640nm.

LEICATCS SP5 MP Multiphoton Imaging Microscope

The Leica TCS can be used to study cells in vivo. The restricted excitation reducesphoto-toxicity and is ideal for investigating living cells, thick specimens,and samples which react sensitive to surrounding properties. Longer excitation wavelengths are less scattered in biological tissues and allow deeper penetration into very thick specimen.

Proteomics Platform

Onthis platform at the intersection of chemistry and biology, the massspectrometers allow protein and peptides characterization, with an emphasis in glycoimmunomics. Researchers resort in this technology to address pathogenesisand clinical questions such as pathogen recognition, autoimmune diseases, genetic disorders and cancer.

Thermo Fisher Trace ISQ GC-MS Agilent 1260 infinity LC

Areas of Expertise

• Proteinseparation and identification

• Structureprediction of proteins and glycans

• Characterization of protein-protein interactions

• Differential protein e­xpression profiles

Cell Sorting Platform

The application of Fluorescent cell sorting is becoming more and more popular in clinical and research areas. Its applications include phenotypic analysis and immune functions of sub-celltypes, nucleic acid analysis to study cells differentiation and apoptosis, molecularcytogenetic, studies of cell functions, as well as microbiological applications.

BDAria III Fluorescence Activating Cell Sorter

The Aria III is a four laser, 13 colour cell sorter. The flow cell is agel-coupled cuvette as opposed to the jet-in-air system, and this combined with a trigon and octagon detection system provide increased sensitivity and resolution.  This instrument can separate any phenotype required in the next step experiment with high purity and very high rate up to 70,000/s.

BDCanto II & Calibur Fluorescence Activating Cell Sorter

Specifications: 2 lasers: 488nm,635nm to do up to 8 color experiments with up to 10,000/sevents rate.

The BD FACSCanto II provides ultimate flexibility and can be configured with two or three lasers to detect multiple colors. The system is an easy-to-use bench top analyzer that delivers proven performance, accuracy, and high-quality results.

Micromanipulation Platform

EppendorfTransferman NK2

Specifications: 40nm/microstep on x-y-z axis; continuous injection; 3-position record.

Applications: micromanipulation of suspension cells; microdissection; nuclear transfer; ES cell transfer; DNA /RNA injection in cells, worms and insects.